What is the half life of transferrin?
Plasma transferrin is an iron-transport protein with a half-life of 8 to 10 days that reflects both protein and iron status. Transferrin increases with iron deficiency and decreases when iron status improves or with protein-energy malnutrition.
What is the oxidation state of iron in transferrin?
+3
When iron is bound to transferrin, the oxidation state is +3. This oxidation state of iron does not change when transferrin is not bound to the metal due to physiological pH. Electron transfer is accomplished by electrons moving from one molecule to another molecule.
What is Apo Transferrin?
Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Apo-transferrin can be loaded with iron prior to use or added directly to an iron containing medium.
What does high soluble transferrin receptor mean?
What does the test result mean? The level of soluble transferrin receptor (sTfR) will be increased with iron deficiency. If you have anemia and your sTfR level is elevated, then it is likely that you have iron deficiency anemia.
What is Apo transferrin?
Why is soluble transferrin receptor elevated in iron deficiency anemia?
Soluble transferrin receptor levels in plasma are elevated if there is increased iron demand due to Iron deficiency, increased erythropoiesis (eg, Haemolysis) or dyserythropoiesis (eg, Megaloblastic anaemia), regardless of other, coexistent states.
What is a normal soluble transferrin receptor?
Normal adult levels vary from 2 to 5 mg/l. However, pediatric studies are few and describe normal ranges to the tune of 1.0–3.0 mg/l, which are relatively lower than that of adults. In present study 40 healthy children (2–12 years) were evaluated to establish normal soluble transferrin receptor range.
What is Apo ferritin?
Apoferritin is a protein commonly present in the intestinal mucosa membrane. The important biological function of apoferritin is its ability to bind and store iron, by combining with a ferric hydroxide–phosphate compound to form ferritin.
Is transferrin different from ferritin?
Transferrin is the main iron transporting protein in the circulation. Ferritin concentrations reflect the body’s iron stores.
How does ferritin distinguish itself from transferrin?
transferrin – measures levels of the iron carrier; total iron binding capacity (TIBC) – measures the transferrin capacity to bind iron; serum ferritin – measures the body’s ability to store iron.
Is ferritin water soluble?
Hemosiderin and ferritin are iron-containing proteins with magnetic susceptibility. Hemosiderin is water-insoluble and thermally denatured, but ferritin is water-soluble and heat-resistant up to 75°C. These characteristic differences were used for the fractionation of ferritin and hemosiderin.
What is the difference between transferrin and apo-transferrin?
Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types.
What is apo transferrin used for?
Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Apo-transferrin can be loaded with iron prior to use or added directly to an iron containing medium. Non-heme iron-transport protein.
Does transferrin bind iron to plasma?
Transferrin has two high-affinity binding sites for iron and exists in plasma as a mixture of apotransferrin and the mono- and diferric forms. Transferrin may be measured either directly by immunological techniques or indirectly by its functional capacity to bind iron.
What is the pathophysiology of iron incorporation into apotransferrin?
Iron incorporation into apotransferrin is facilitated by the oxidation of Fe2+ to Fe 3+ by hephaestin, a member of the family of multicopper oxidases (which includes ceruloplasmin) which appears to be bound to the enterocyte basolateral membrane. Figure 7.21. Iron import, utilization and export pathways according to cell type. (A) Enterocyte.