What does the Sendai virus do?
Sendai virus is used as a backbone for vaccine development against Mycobacterium tuberculosis that causes tuberculosis, against HIV-1 that causes AIDS and against other viruses, including those that cause severe respiratory infections in children.
Is Sendai virus episomal?
They reprogrammed human fibroblasts using a Sendai virus–based system, an episomal system, or mRNA-based reprogramming, benchmarking these to integrating viral methods.
How does Sendai virus reprogram cells?
The Sendai virus vectors contain transgenes that will express factors hOct3/4, hSox2, hKlf4, and hc-Myc. After transduction, the viral vectors will cause the cells to express these four genes, resulting in reprogramming.
What is Sendai in rats?
Sendai virus is among a very few naturally occurring viruses that can cause severe respiratory disease with high mortality in adult mice and, to a much lesser extent, in rats and other laboratory animals. Sendai virus is highly contagious among rodents.
What is Newcastle disease virus?
Newcastle disease virus (NDV) is a virus that causes a deadly infection in many kinds of birds. In humans, NDV causes mild flu-like symptoms or conjunctivitis (an infection of the eye that is also called pink eye) and/or laryngitis (an irritation and swelling of the voice box and the area around it).
Is Sendai virus a lentivirus?
What is SDA disease?
SDA has a high morbidity but usually a low mortality with mild clinical signs: squinting, photophobia, blinking, sneezing, and/or swelling under the neck caused by either edema, enlarged cervical lymph node, or inflamed salivary glands.
What is SDA virus in rats?
Sialodacryoadenitis (SDA) is a common short-lived acute infection in rats caused by a coronavirus, and is akin to mumps in humans. It is highly contagious among rats and spreads by the respiratory route .
Can a bird recover from Newcastle disease?
Newcastle disease, also called avian pneumoencephalitis, a serious viral disease of birds caused by a paramyxovirus and marked by respiratory and nervous system problems. Some adult birds recover, although mortality rates are high in tropical and subtropical regions.
Can garlic treat Newcastle?
However, 0.4-0.6% garlic supplementation in drinking water was effective to reduce mortality and enhance immunity against Newcastle disease virus in vaccinated broiler chickens against Newcastle disease.
What does Yamanaka factors do?
The Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) are a group of protein transcription factors that play a vital role in the creation of induced pluripotent stem cells (cells that have the ability to become any cell in the body), often called iPSCs. They control how DNA is copied for translation into other proteins.
What kind of scientist is Shinya Yamanaka?
Shinya Yamanaka, Japanese Yamanaka Shinya, (born September 4, 1962, Ōsaka, Japan), Japanese physician and researcher who developed a revolutionary method for generating stem cells from existing cells of the body.
What did Shinya Yamanaka study?
Shinya Yamanaka was born in Higashiosaka, Japan. He studied for his medical degree at Kobe University and later earned his PhD from Osaka City University in 1993.
What is the pathogenesis of Sendai virus infection in genetically resistant mice?
The pathogenesis of Sendai virus infection in genetically resistant (C57Bl/6) and susceptible (DBA/2) nonimmune adult mice was investigated. Rising serum complement-fixation (CF) antibody titers were delayed in DBA/2 mice as compared with C57Bl/6 mice.
Can ts12kos be used to eliminate Sendai virus?
Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells.
What is the incubation protocol for Sendai virus infection?
The cultures with new Sendai virus infection were incubated at 36°C for one week. From 18 to 25 days after infection, colonies were picked up and re-cultured again in the iPS cell medium. In some experiments, FGF2 concentration was modified from 5 ng/ml to 30 ng/ml and NaB was added on day 2 in the iPSC induction.
What are the competing interests of HB and NF in dnavec?
Competing interests: HB is an employee of DNAVEC Corporation. M. Hasegawa is a founder and adviser of DNAVEC Corporation. NF was an employee of DNAVEC Corporation until January 2013 but not now. The commercial product developed by DNAVEC Corporation is similar to the vectors described in this paper but the component is different.