## What does a Lineweaver-Burk plot show?

The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents −1/Km.

## What does the slope of a Lineweaver-Burk plot represent?

On the graph, the x-intercept can be used to determine the Michaelis constant, the y-intercept can be used to determine the maximal velocity and the slope represents the ratio of the Michaelis constant to the maximal velocity.

**How would you determine Vmax using a Lineweaver-Burk plot?**

Ease of Calculating the Vmax in Lineweaver-Burk Plot Since the slope-intercept equation relates the rate to the concentration of the substrate, you can use the typical formula of y = mx + b, where y is the dependent variable, m is the slope, x is the independent variable, and b is the y-intercept.

**Why is the Lineweaver-Burk plot important in enzyme kinetics?**

Why is the Lineweaver-Burk plot important in enzyme kinetics? – It is a single-reciprocal plot. – It illustrates enzyme specificity. – It reveals the presence of prosthetic groups in enzymes.

### How do you determine inhibition from a Lineweaver-Burk plot?

As shown in Figure 13.14, when we display kinetic data using as a Lineweaver-Burk plot it is easy to determine which mechanism is in effect. For example, an increase in slope, a decrease in the x-intercept, and no change in the y-intercept indicates competitive inhibition.

### How do you determine the type of inhibition from a Lineweaver-Burk plot?

**What are the advantages of Lineweaver-Burk plot?**

For instance; Lineweaver-Burke plot, the most favoured plot by researchers, has two distinct advantages over the Michaelis-Menten plot, in that it gives a more accurate estimate of Vmax and more accurate information about inhibition. It increases the precision by linearizing the data.

**Why is the Lineweaver Burk plot important in enzyme kinetics?**

## How can you tell if an inhibitor is competitive or noncompetitive?

Competitive vs. noncompetitive

- If an inhibitor is competitive, it will decrease reaction rate when there’s not much substrate, but can be “out-competed” by lots of substrate.
- If an inhibitor is noncompetitive, the enzyme-catalyzed reaction will never reach its normal maximum rate even with a lot of substrate.

## What does Km Tell us about the enzyme?

It indicates the affinity of an enzyme for a given substrate: the lower the KM value, the higher the affinity of the enzyme for the substrate.

**Why is Lineweaver plot more accurate?**

**How do you determine the type of inhibition from a Lineweaver Burk plot?**

### What does it mean if Vmax decreases?

Decreases in free enzyme correspond to an enzyme with greater affinity for its substrate. Thus, paradoxically, uncompetitive inhibition both decreases Vmax and increases an enzyme’s affinity for its substrate.

### How does the Lineweaver Burk plot change during uncompetitive inhibition with increasing inhibitor concentration?

For uncompetitive inhibition, the intercept of the Lineweaver-Burk plots is increased, and the Ki value is designated by Kii, where the second i refers to the intercept. With noncompetitive inhibition, there are both slope and intercept effects.

**What is an example of a Lineweaver Burk plot?**

An example of a Lineweaver-Burk plot. In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934.

**What is the y-intercept of the Lineweaver-Burk plot?**

The y-intercept of the Lineweaver- Burk plot is the reciprocal of maximum velocity. K M: Michaelis-Menten constant or enzyme affinity.

## What is the independent axis of the Lineweaver-Burk plot?

The independent axis of Lineweaver- Burk plot is the reciprocal of substrate concentration. V 0 or V: initial velocity of an enzyme inhibited reaction. The dependent axis of the Lineweaver- Burk plot is the reciprocal of velocity.

## Why is the Lineweaver Burk plot used for enzyme kinetics?

While the Lineweaver-Burk is useful for determining important variables in enzyme kinetics, it is prone to error. The y-axis of the plot takes the reciprocal of the rate of reaction, meaning small errors in measurement are more noticeable.