Is Lactophenol cotton blue toxic?
Ingestion Harmful if swallowed. May cause chemical burns in mouth, oesophagus and stomach. Skin contact This product is strongly corrosive. May cause serious chemical burns to the skin.
What are the potential hazards of Lactophenol cotton blue?
Hazard statements H302+H332 Harmful if swallowed or if inhaled. H314 Causes severe skin burns and eye damage. H341 Suspected of causing genetic defects. H373 May cause damage to organs through prolonged or repeated exposure.
What are the hazards of methylene blue?
May cause discomfort. Ingestion May cause discomfort if swallowed. Skin contact Causes mild skin irritation. Prolonged contact may cause redness, irritation and dry skin.
Is methylene blue stain toxic?
Methylene Blue (61-73-4) Skin and eye contact. Potential Adverse human health effects and symptoms : Harmful if swallowed. Slightly irritant to eyes. Symptoms/effects after skin contact : May stain the skin.
How do you stain fungi with Lactophenol cotton blue?
Immerse the specimen/material in the drop of alcohol. Add one, or at most two drops of the lactophenol/cotton blue mountant/stain before the alcohol dries out. Holding the coverslip between forefinger and thumb, touch one edge of the drop of mountant with the coverslip edge, and lower gently, avoiding air bubbles.
Is there any alternative for lactophenol cotton blue?
Iodine-glycerol as an alternative to lactophenol cotton blue for identification of fungal elements in clinical laboratory.
Why is Lactophenol blue used?
Lactophenol blue solution is used to visualise fungal cells in various cytological materials.
Why lactophenol cotton blue is used in staining fungi?
Lactophenol cotton blue is a stain that is used to examine fungal elements following either a tape preparation or a scraping. This stain contains phenol, which will kill the organisms, lactic acid which preserves fungal structures, and cotton blue which stains the chitin found in the fungal cell walls.
What is the role of the phenol in lactophenol cotton blue?
The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The solution is clear and blue in color and it is made up of a combination of three main reagents: Phenol: It acts as a disinfectant by killing any living organisms. Lactic acid: To preserve the fungal structures.
What is Lactophenol cotton blue stain?
Lactophenol Blue Solution or Lactophenol cotton blue is a wet mounting medium and staining agent which is used for the preparation of microscopic slides of fungi for examination. Fungal elements are stained intensely blue.
Why does Lactophenol cotton blue affect the cell wall?
Principle of Lactophenol Cotton Blue (LPCB) Staining The fungal spore cell wall is made up of chitin of which the components of the Lactophenol Cotton Blue solution stains for identification. The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent.
Can you take methylene blue orally?
For use as a diagnostic aid to determine the presence of a gastrointestinal fistula (e.g., gastrointestinal fistula diagnosis). In patients with a suspected gastrointestinal fistula, a small quantity (e.g., 5—10 ml) of methylene blue can be administered either orally or via a nasogastric tube.
Is methylene chloride a human carcinogen?
Because methylene chloride has been shown to induce increased numbers of benign and malignant neoplasms in rats and mice, it meets the criteria provided in the OSHA Cancer Policy for classifying a substance as a potential occupational carcinogen; therefore, NIOSH recommends that methylene chloride be considered a …
What is the purpose of Lactophenol blue?
Why does lactophenol cotton blue affect the cell wall?
Why is Lactophenol cotton blue used?
Lactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.